Differences in the number of nucleolus organizers in Chironomus tepperi shown by in situ hybridization |
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Authors: | Jürgen Eigenbrod |
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Institution: | (1) Lehrstuhl für Genetik, MA 5, Ruhr-Universität Bochum, Postfach 102148, D-4630 Bochum, Germany |
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Abstract: | Two stocks of Chironomus tepperi could be isolated. One stock, N(IV)+, contains nucleolus organizers in chromosome I and IV, whereas the other one, N(IV)–, shows only one nucleolus in chromosome I. It is demonstrated by in situ hybridization with radioactive rRNA that the absence of the nucleolus in chromosome IV of stock N(IV)– is not related to an inactivation of the nucleolar DNA, as might have been suggested, but is due to the lack of ribosomal cistrons. |
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