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Synchronous culture of Magnetospirillum sp. AMB-1 by repeated cold treatment |
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| Authors: | Rika Sato Toko Miyagi Shinji Kamiya Toshifumi Sakaguchi Richard H. Thornhill Tadashi Matsunaga |
| Affiliation: | TDK Akita Laboratory Corporation, 15 Aza-Gashomen, Hirasawa, Nikaho-machi, Yuri-gun, Akita 018-04, Japan; R &D Center, TDK Corporation, 2-15-7, Higashi-owada, Ichikawa-shi, Chiba 272, Japan; Department of Biotechnology, Tokyo University of Agriculture &Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184, Japan |
| Abstract: | Abstract We established a synchronous culture of Magnetospirillum sp. AMB-1 by repeated cold treatment at 5 °C. This is the first reported synchronous culture of a magnetic bacterium. Cold treatment did not affect magnetic particle synthesis or cell morphology. Iron uptake was observed both before and during cell division. The amount of iron uptake was almost equivalent to that of magnetite formation. The proportion of magnetosensitive cells did not change during cell division. |
| Keywords: | Synchronous culture Cold treatment Magnetospirillum sp. AMB-1 Magnetosensitivity Iron uptake Magnetic bacteria |