Development and characterization of microsatellite markers for Prunus verecunda and Prunus grayana (Rosaceae) |
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| Authors: | Ayako Sawa Shingo Kaneko Yuji Isagi Shigeru Mariko Takashi Masaki |
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| Affiliation: | (1) Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan;(2) Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho Sakyo-ku, Kyoto 606-8502, Japan;(3) Hosei University, Machida, Tokyo 194-0298, Japan;(4) Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan |
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| Abstract: | Simple sequence repeat (SSR) markers were developed for Prunus verecunda and Prunus grayana to help understand the seed dispersal pattern of each species. We isolated and characterized nine microsatellite loci (four from P. verecunda and five from P. grayana). In P. verecunda, the number of alleles detected and the expected heterozygosity of five loci ranged from 11 to 24 and 0.59 to 0.92, respectively. In P. grayana, the number of alleles detected and the expected heterozygosity of five loci ranged from 4 to 14 and 0.62 to 0.86, respectively. These results show that the markers described here will be useful in studying the seed dispersal pattern of Prunus species. |
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