Molecular cloning and expression profile of a jasmonate biosynthetic pathway gene for allene oxide cyclase from <Emphasis Type="Italic">Hyoscyamus niger</Emphasis> |
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| Authors: | Keji Jiang Zhihua Liao Yan Pi Zhuoshi Huang Rong Hou Ying Cao Qing Wang Xiaofen Sun Kexuan Tang |
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| Institution: | (1) State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&;D Center, Fudan University, Shanghai, 200433, People’s Republic of China;(2) Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai, 200433, People’s Republic of China;(3) Laboratory of Plant Bioengineering, College of Life and Environment, Shanghai Normal University, Shanghai, 200234, People’s Republic of China;(4) Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, People’s Republic of China;(5) Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&;D Center, Institute of Systems Biology, Shanghai Jiao Tong University, Shanghai, 200030, People’s Republic of China |
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| Abstract: | Hyoscyamus niger L. is a medicinal plant which produces a class of jasmonate-responsive pharmaceutical secondary metabolites named tropane
alkaloids. As a family of signaling phytohormones, jasmonates play significant roles in the biosynthesis of many plant secondary
metabolites. In the jasmonate biosynthetic pathway of plants, allene oxide cyclase (AOC, EC 5.3.99.6) catalyzes the most important
step. Here we cloned a cDNA from H. niger, named HnAOC (GenBank accession no.: AY708383), which was 1044 bp long, with a 747-bp open reading frame (ORF) encoding a polypeptide
of 248 amino acid residues. Southern blot analysis indicated that it was a multicopy gene. RT-PCR analysis revealed that the
expression of HnAOC was regulated by various stresses and elicitors, with methyl-jasmonate showing the most prominent inducement. The characterization
of HnAOC would be helpful for improving the production of valuable secondary metabolites by regulating the biosynthesis of jasmonates.
The text was submitted by the authors in English. |
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| Keywords: | allene oxide cyclase Hyoscyamus niger jasmonate RT-PCR secondary metabolite |
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