Phospholipid dependence of calcium ion effects on electrophoretic mobilities of liposomes |
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Authors: | J W Klein B R Ware G Barclay H R Petty |
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Affiliation: | 1. Department of Chemistry, Syracuse University, Syracuse, NY 13244-1200 USA;2. Department of Biological Sciences, Wayne State University, Detroit, MI 48202 U.S.A. |
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Abstract: | Electrophoretic light scattering (ELS) and depolarization of fluorescence have been used to determine the effect of membrane fluidity on the binding of Ca2+ to liposomes. ELS was used to measure the electrophoretic mobilities of the liposomes. Fluorescence depolarization was used to determine membrane fluidity. Zero to 30 mol% phosphatidylserine (PS) was incorporated into liposomes containing, as bulk phospholipids, one of the following: dimyristoyl-phosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), egg phosphatidylcholine (PC), or hydrogenated egg phosphatidylcholine (H egg PC). The binding of Ca2+ to the liposomes appears to be influenced by membrane fluidity. Liposomes containing bulk phospholipids whose phase transition temperature is higher than the experimental temperature exhibit enhanced binding of CA2+. |
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