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In vitro culture of Foeniculum vulgare: callus characteristics in relation to morphogenesis
Authors:M. Anzidei  L. Vivona  S. Schiff  A. Bennici
Affiliation:(1) Plant Biology Department, University of Florence, P. le Cascine, 28-50144 Florence, Italy
Abstract:Callus induction and morphogenic response of several fennel populations were determined by genotype and hormonal treatment. 100% callus formation occurred only in the Francia Pernod population under the action of 2, 4-dichlorophenoxyacetic acid or agr-naphthaleneacetic acid and kinetin. Only this last hormonal treatment induced shoot regeneration. Plant regeneration was observed especially in Francia Pernod population. Ths calluses grown in presence of 2,4-dichlorophenoxyacetic acid or agr-naphthaleneacetic acid plus kinetin, showed considerable differences at cytological and histological level which were correlated to their different morphogenic capability.Abbreviations 2, 4-d 2,4-dichlorophenoxyacetic acid - MS Murashige and Skoog medium - NAA agr-naphthaleneacetic acid
Keywords:Auxin  callus  cytokinin  fennel  histological analysis  plant regeneration
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