In vitro culture of Foeniculum vulgare: callus characteristics in relation to morphogenesis

Callus induction and morphogenic response of several fennel populations were determined by genotype and hormonal treatment. 100% callus formation occurred only in the Francia Pernod population under the action of 2, 4-dichlorophenoxyacetic acid or -naphthaleneacetic acid and kinetin. Only this last hormonal treatment induced shoot regeneration. Plant regeneration was observed especially in Francia Pernod population. Ths calluses grown in presence of 2,4-dichlorophenoxyacetic acid or -naphthaleneacetic acid plus kinetin, showed considerable differences at cytological and histological level which were correlated to their different morphogenic capability.Abbreviations 2, 4-d 2,4-dichlorophenoxyacetic acid - MS Murashige and Skoog medium - NAA -naphthaleneacetic acid