Cerebral gene expression and neurobehavioural development after perinatal exposure to an environmentally relevant polybrominated diphenylether (BDE47) |
| |
Authors: | Marte Haave Kristin Ingvaldsen Folven Thomas Carroll Chris Glover Einar Heegaard Trond Brattelid Christer Hogstrand Anne-Katrine Lundebye |
| |
Institution: | (1) National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029, Nordnes, 5817 Bergen, Norway;(2) Department of Biology, University of Bergen, PO box 7803, 5020 Bergen, Norway;(3) Nutritional Sciences Division, King’s College London, Franklin-Wilkins Building, 150 Stamford Street, London, SE1 9NH, UK;(4) Norwegian Forest and Landscape Institute, Fanaflaten 4, 5244 Fana, Norway;(5) Present address: University of Canterbury, Christchurch, New Zealand |
| |
Abstract: | Nutrients in seafood are known to be beneficial for brain development. Effects of maternal exposure to 2,2′,4,4′ tetrabromo
diphenylether (BDE47) was investigated, alongside the potential ameliorating impact of seafood nutrients, through assessment
of neurobehaviour and gene expression in brain and liver. Developing mice were exposed during gestation and lactation via
dams dosed through casein- or salmon-based feed, spiked with BDE47. Two concentrations were used: a low level (6 μg/kg feed)
representing an environmentally realistic concentration and a high level (1,900 μg/kg feed) representing a BDE47 intake much
higher than expected from frequents consumption of contaminated seafood. Experimental groups were similar with respect to
reproductive success, growth and physical development. Minor, transient changes in neurobehavioural metrics were observed
in groups given the highest dose of BDE47. No significant differences in behaviour or development were seen on postnatal day 18
among maternally exposed offspring. Cerebral gene expression investigated by microarray analyses and validated by RT-qPCR
showed low fold changes for all genes, despite dose-dependent accumulation of BDE47 in brain tissue. The gene for glutamate
ammonia ligase was upregulated compared to control in the casein-based high BDE47diet, suggesting potential impacts on downstream
synaptic transmission. The study supported a previously observed regulation of Igfbp2 in brain with BDE47 exposure. Genes for hepatic metabolic enzymes were not influenced by BDE47. Potential neurotoxic effects
and neurobehavioural aberrations after perinatal exposure to high levels of BDE47 were not readily observed in mice pups with
the present experimental exposure regimes and methods of analysis. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|