Association mapping of quantitative resistance for <Emphasis Type="Italic">Leptosphaeria maculans</Emphasis> in oilseed rape (<Emphasis Type="Italic">Brassica napus</Emphasis> L.) |
| |
Authors: | C Jestin M Lodé P Vallée C Domin C Falentin R Horvais S Coedel M J Manzanares-Dauleux R Delourme |
| |
Institution: | 1.INRA, Agrocampus Ouest, Université Rennes 1,Le Rheu Cedex,France |
| |
Abstract: | Stem canker caused by the fungus Leptosphaeria
maculans is a major disease of Brassica
napus. Quantitative resistance factors appear to be important components for effective and durable control of this pathogen. Quantitative
trait loci (QTL) for stem canker resistance have previously been identified in the Darmor variety. However, before these QTL
can be used in marker-assisted selection (MAS) to breed resistant varieties, they must be validated in a wide range of genetic
backgrounds. We used an association mapping approach to confirm the markers located within the QTL previously identified in
Darmor and establish their usefulness in MAS. For this, we characterized the molecular diversity of an oilseed rape collection
of 128 lines showing a large spectrum of responses to infection by L. maculans, using 72 pairs of primers for simple sequence repeat and other markers. We used different association mapping models which
either do or do not take into account the population structure and/or family relatedness. In all, 61 marker alleles were found
to be associated with resistance to stem canker. Some of these markers were associated with previously identified QTL, which
confirms their usefulness in MAS. Markers located in regions not harbouring previously identified QTL were also associated
with resistance, suggesting that new QTL or allelic variants are present in the collection. All of these markers associated
with stem canker resistance will help identify accessions carrying desirable alleles and facilitate QTL introgression. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|