Microsatellite mapping of <Emphasis Type="Italic">Ae. speltoides</Emphasis> and map-based comparative analysis of the S,G, and B genomes of Triticeae species |
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Authors: | O Dobrovolskaya C Boeuf J Salse C Pont P Sourdille M Bernard E Salina |
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Institution: | 1.Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences,Novosibirsk,Russia;2.UMR INRA-UBP Génétique, Diversité et Ecophysiologie des Céréales,Clermont-Ferrand Cedex 2,France |
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Abstract: | The first microsatellite linkage map of Ae. speltoides Tausch (2n = 2x = 14, SS), which is a wild species with a genome closely related to the B and G genomes of polyploid wheats, was developed
based on two F2 mapping populations using microsatellite (SSR) markers from Ae. speltoides, wheat genomic SSRs (g-SSRs) and EST-derived SSRs. A total of 144 different microsatellite loci were mapped in the Ae. speltoides genome. The transferability of the SSRs markers between the related S, B, and G genomes allowed possible integration of new
markers into the T. timopheevii G genome chromosomal maps and map-based comparisons. Thirty-one new microsatellite loci assigned to the genetic framework
of the T. timopheevii G genome maps were composed of wheat g-SSR (genomic SSR) markers. Most of the used Ae. speltoides SSRs were mapped onto chromosomes of the G genome supporting a close relationship between the G and S genomes. Comparative
microsatellite mapping of the S, B, and G genomes demonstrated colinearity between the chromosomes within homoeologous groups,
except for intergenomic T6AtS.1G, T4AL.5AL.7BS translocations. A translocation between chromosomes 2 and 6 that is present in the T. aestivum B genome was found in neither Ae. speltoides nor in T. timopheevii. Although the marker order was generally conserved among the B, S, and G genomes, the total length of the Ae. speltoides chromosomal maps and the genetic distances between homoeologous loci located in the proximal regions of the S genome chromosomes
were reduced compared with the B, and G genome chromosomes. |
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