Ethylene-binding activity, gene expression levels, and receptor system output for ethylene receptor family members from Arabidopsis and tomato |
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Authors: | O'Malley Ronan C Rodriguez Fernando I Esch Jeffrey J Binder Brad M O'Donnell Philip Klee Harry J Bleecker Anthony B |
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Affiliation: | Department of Botany, University of Wisconsin, 430 Lincoln Drive, Madison, WI 53706, USA. |
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Abstract: | Ethylene signaling in plants is mediated by a family of ethylene receptors related to bacterial two-component regulators. Expression in yeast of ethylene-binding domains from the five receptor isoforms from Arabidopsis thaliana and five-receptor isoforms from tomato confirmed that all members of the family are capable of high-affinity ethylene-binding activity. All receptor isoforms displayed a similar level of ethylene binding on a per unit protein basis, while members of both subfamily I and subfamily II from Arabidopsis showed similar slow-release kinetics for ethylene. Quantification of receptor-isoform mRNA levels in receptor-deficient Arabidopsis lines indicated a direct correlation between total message level and total ethylene-binding activity in planta. Increased expression of remaining receptor isoforms in receptor-deficient lines tended to compensate for missing receptors at the level of mRNA expression and ethylene-binding activity, but not at the level of receptor signaling, consistent with specialized roles for family members in receptor signal output. |
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Keywords: | ethylene receptor signal transduction plant hormone membrane-associated receptor ETR1 |
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