Yeast Artificial Chromosome Clones of Rice Chromosome 2 Ordered Using DNA Markers |
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| Authors: | Umehara Yosuke; Kurata Nori; Ashikawa Ikuo; Sasaki Takuji |
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| Institution: | Rice Genome Research Program, National Institute of Agrobiological Resources 1-2 Kannondai, 2-chome, Tsukuba, Ibaraki 305, Japan/Institute of Society for Techno-Innovation of Agriculture, Forestry and Fisheries 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305, Japan |
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| Abstract: | Yeast artificial chromosome (YAC) clones were ordered for thephysical mapping of rice chromosome 2, the last of the 12 ricechromosomes to be assigned YACs by the Rice Genome ResearchProgram. A total of 128 restriction fragment length polymorphismmarkers and 4 sequence-tagged site (STS) markers located onour high-density genetic map were used for YAC clone landing.By colony/Southern hybridization and polymerase chain reactionscreening, a total of 239 individual YACs were selected fromour YAC library of 6934 clones covering six genome equivalents.The YACs located on the corresponding marker positions in thelinkage map formed 43 contigs and islands and were estimatedto encompass about 50% of the length of rice chromosome 2. |
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| Keywords: | yeast artificial chromosome (YAC) physical mapping rice |
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