7-ketocholesterol inhibits VLDL secretion by cultured human and rabbit hepatocytes |
| |
| Authors: | V A Kosykh E A Podres A B Sudarickov Y V Berestetskaya A P Surguchov V S Repin V N Smirnov |
| |
| Affiliation: | U.S.S.R. Cardiology Research Center, Academy of Medical Sciences, Moscow. |
| |
| Abstract: | 7-ketocholesterol, one of the major product of autoxidation of dietary cholesterol, was found to inhibit secretion of very low density lipoprotein [14C]cholesterol, [14C]triacylglycerol and [35S]apoprotein B,E,C by cultured human and rabbit hepatocytes. A parallel inhibition (about 35%) of cholesterol synthesis but not of triacylglycerol formation was observed. Incubation with 10 micrograms/ml of oxysterol also reduced the total apo-B secretion measured by ELISA and increased intracellular apo-B mRNA level. These results seem to indicate that 7-ketocholesterol decreases secretion of very low density lipoprotein (VLDL) particles and exerts inhibitory effects on apo-B production at the co-translational or posttranslational level. |
| |
| Keywords: | |
|
|
