7-ketocholesterol inhibits VLDL secretion by cultured human and rabbit hepatocytes |
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| Authors: | V A Kosykh E A Podres A B Sudarickov Y V Berestetskaya A P Surguchov V S Repin V N Smirnov |
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| Institution: | U.S.S.R. Cardiology Research Center, Academy of Medical Sciences, Moscow. |
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| Abstract: | 7-ketocholesterol, one of the major product of autoxidation of dietary cholesterol, was found to inhibit secretion of very low density lipoprotein 14C]cholesterol, 14C]triacylglycerol and 35S]apoprotein B,E,C by cultured human and rabbit hepatocytes. A parallel inhibition (about 35%) of cholesterol synthesis but not of triacylglycerol formation was observed. Incubation with 10 micrograms/ml of oxysterol also reduced the total apo-B secretion measured by ELISA and increased intracellular apo-B mRNA level. These results seem to indicate that 7-ketocholesterol decreases secretion of very low density lipoprotein (VLDL) particles and exerts inhibitory effects on apo-B production at the co-translational or posttranslational level. |
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