Chromosome mapping in Alealigenes eutrophus CH34 |
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Authors: | Abdelkrim Sadouk and Max Mergeay |
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Institution: | (1) Laboratoire de Génétique et Biotechnologie, Centre d'Etudes de l'Energie Nucléaire and Vlaamse Instelling voor Technologisch Onderzoek, SCK/CEN-VITO, B-2400 Mol, 200 Boeretang, Belgium |
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Abstract: | Mutants and mobilizing plasmids were developed as genetic tools in Alcaligenes eutrophus CH34. In order to map the chromosome, spontaneous and ethyl methane sulphonate (EMS)-induced mutants (mostly auxotrophs) were isolated. Another source of mutants was provided by the phenomenon of temperature-induced mortality and mutagenesis that is observed at 37° C and is characteristic of many metallotolerant strains of A. eutrophus. Plasmid pULB113 (RP4::miniMu) was used to map the available mutations. Twenty-five loci were ordered in a circular map. pMOL50, a rearranged derivative of plasmid pMOL28, which was obtained in a survivor at 37° C and displayed chromosome mobilizing activity (Cma+), was also used to mobilize chromosomal markers: resulting linkages were stronger than with pULB113, allowing confirmation of the circularity of the A. eutrophus CH34 chromosome with a small number of crosses. |
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Keywords: | Chromosome mapping Conjugative plasmids Alcaligenes eutrophus |
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