Immobilization of glucosyltransferase fromAureobasidium |
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Authors: | Dr Sachio Hayashi Yasuhiro Ueda Goichi Kobayashi Yoshiyuki Takasaki Kiyohisa Imada |
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Institution: | (1) Department of Materials Science, Faculty of Engineering, Miyazaki University, 1-1 Gakuen Kibanadai Nishi, 889-21 Miyazaki, Japan |
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Abstract: | Summary Glucosyltransferase fromAureobasidium, which produces panose and isomaltose from maltose, was immobilized by alginate gel or DEAE-cellulose at high efficiency (71 and 41% respectively). Alkylamine porous silica was less efficient as a support. The enzymatic profiles of immobilized enzymes were almost identical to the native one except that their stabilities to extreme pH, metal ions and inhibitors were improved. Both immobilization procedures successfully produced high amounts of panose, 125 mg ml–1 (alginate gel) or 141 mg ml–1 (DEAE-cellulose), from 300 mg ml–1 of maltose. |
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Keywords: | 1 4- -d-Glucan 6- gif" alt="agr" align="BASELINE" BORDER="0">-d-Glucan 6- -d-glucosyltransferase" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">-d-glucosyltransferase Panose Isomaltose Maltose |
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