Involvement of Elk-1 in L6E9 skeletal muscle differentiation |
| |
Authors: | Khurana Ashwani Dey Chinmoy S |
| |
Institution: | Department of Cell Biology, Wenner Gren Institute, Stockholm University, Sweden. |
| |
Abstract: | The binding of phosphatidylinositol(4,5)-bisphosphate (PI(4,5)P(2)) to profilin at a region distinct from the actin interaction surface is demonstrated by experiments with covalently cross-linked profilin:beta-actin. The result is in agreement with observations made with several mutant profilins and provides strong evidence for two regions on mammalian profilin mediating electrostatic interaction with phosphatidylinositol lipids; one close to the binding site for poly(L-proline), and one partially overlapping with the actin-binding surface. Congruent with this, two plant profilins, which have a reduced number of positive amino acids in one of these regions, displayed a dramatically lower binding to PI(4,5)P(2) compared to human profilin I. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|