In vitro study of gas effects on alveolar macrophages |
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Authors: | B Wallaert C Voisin |
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Institution: | (1) Expérimentale and CJF INSERM, Institut Pasteur, Laboratoire de Pollution Atmosphérique et de Pathologie Respiratoire, Lille, France;(2) Present address: Laboratoire de Pollution atmosphérique, Institut Pasteur de Lille, 1, rue Calmette, 59800 Lille, France |
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Abstract: | Summary To evaluate the biological effects of gas pollutants on alveolar macrophages several in vitro systems ave been developed. We described here an original method of cell culture in aerobiosis, which permitted direct contact between the atmosphere and the target cells. We studied the long term (24 h) and short term (30 min) effects of NO2 on alveolar macrophages. Our results demonstrated that exposure of alveolar macrophages to gas pollutants may be responsible for either cell injury or cell activation associated with the release of various bioactive mediators (superoxide anion, neutrophil chemotactic activity). Cell culture in aerobiosis opens new ways for the research on the biological effects of gas pollutants.Abbreviations AM
alveolar macrophages
- CL
Chemiluminescence |
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Keywords: | alveolar macrophages gases in vitrosystems oxidants |
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