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A single nucleotide substitution in TaHKT1;5-D controls shoot Na+ accumulation in bread wheat |
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| Authors: | Chana Borjigin Rhiannon K. Schilling Jayakumar Bose Maria Hrmova Jiaen Qiu Stefanie Wege Apriadi Situmorang Caitlin Byrt Chris Brien Bettina Berger Matthew Gilliham Allison S. Pearson Stuart J. Roy |
| Affiliation: | 1. Australian Centre for Plant Functional Genomics, The University of Adelaide, Glen Osmond, South Australia, Australia School of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, South Australia, Australia;2. Australian Centre for Plant Functional Genomics, The University of Adelaide, Glen Osmond, South Australia, Australia;3. School of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, South Australia, Australia ARC Centre of Excellence in Plant Energy Biology, The University of Adelaide, Glen Osmond, South Australia, Australia;4. School of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, South Australia, Australia;5. Division of Plant Sciences, Research School of Biology, Australian National University, Acton, Australian Capital Territory, Australia;6. School of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, South Australia, Australia Australian Plant Phenomics Facility, The Plant Accelerator, The University of Adelaide, Glen Osmond, South Australia, Australia;7. Australian Centre for Plant Functional Genomics, The University of Adelaide, Glen Osmond, South Australia, Australia School of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, South Australia, Australia ARC Centre of Excellence in Plant Energy Biology, The University of Adelaide, Glen Osmond, South Australia, Australia |
| Abstract: | Improving salinity tolerance in the most widely cultivated cereal, bread wheat (Triticum aestivum L.), is essential to increase grain yields on saline agricultural lands. A Portuguese landrace, Mocho de Espiga Branca accumulates up to sixfold greater leaf and sheath sodium (Na+) than two Australian cultivars, Gladius and Scout, under salt stress in hydroponics. Despite high leaf and sheath Na+ concentrations, Mocho de Espiga Branca maintained similar salinity tolerance compared to Gladius and Scout. A naturally occurring single nucleotide substitution was identified in the gene encoding a major Na+ transporter TaHKT1;5-D in Mocho de Espiga Branca, which resulted in a L190P amino acid residue variation. This variant prevents Mocho de Espiga Branca from retrieving Na+ from the root xylem leading to a high shoot Na+ concentration. The identification of the tissue-tolerant Mocho de Espiga Branca will accelerate the development of more elite salt-tolerant bread wheat cultivars. |
| Keywords: | Na+ transport plant growth salt tolerance sodium xylem sap Na+ |