Genetic identification of 91 poplar cultivars based on SSR markers |
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Authors: | Pei Sun Huixia Jia Zhiqiang Yue Jinhua Li Jianbo Li Jin Zhang Mengzhu Lu Jianjun Hu |
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Affiliation: | 1. State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Inst. of Forestry, Chinese Academy of Forestry, Xiangshan street, Haidian district, Beijing, PR China;2. State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Inst. of Forestry, Chinese Academy of Forestry, Xiangshan street, Haidian district, Beijing, PR China Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry Univ., Nanjing, PR China |
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Abstract: | Accurate genetic identification and relationship analysis of poplar cultivars is necessary to establish commercial poplar plantations and select suitable breeding strategies. In this study, 91 poplar cultivars belonging to four sections (Aigeiros, Tacamahaca, Populus and Turanga) and inter/intra-sectional hybrids were genotyped using 18 polymorphic simple sequence repeat (SSR) markers. In total, 222 alleles were amplified with an average of 12.3 alleles per marker. The mean polymorphic information content and power of discrimination were 0.706 and 0.813, respectively. Five SSR markers (ORPM_103, ORPM_247, GCPM_1048, GCPM_1255 and LG_X_19) constituted a core fingerprint and were sufficient to identify all the tested cultivars. With some notable exceptions, cultivars of the same species generally clustered together in cluster (UPGMA) and ordination (PCO) analyses. Flow cytometry indicated that 11 poplar cultivars were triploid. Among these, seven had three alleles at some loci, suggesting that SSR markers could indicate the ploidy level to some extent. This study provides useful genetic information for the identification and protection of poplar cultivars in China and offers a guideline for the selection of poplar crossing parents based on ploidy level and genetic relationships. |
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Keywords: | fingerprint flow cytometry Populus triploid UPGMA analysis |
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