针刺百会穴对产后抑郁小鼠行为学改变和海马区NMDAR相关蛋白表达的影响

摘要 目的：研究针刺百会穴对产后抑郁小鼠行为学改变和海马区N-甲基-D-天冬氨酸受体（NMDAR）相关蛋白表达的影响。方法：30只C57BL/6母鼠被随机分为对照组、模型组和治疗组，每组30只。模型组和治疗组小鼠在妊娠期间通过皮下注射地塞米松磷酸钠建立产后抑郁小鼠模型，对照组小鼠皮下注射等量的生理盐水作为对照。治疗组小鼠分娩后通过针刺百会穴治疗14天，模型组和对照组小鼠不进行治疗。比较三组小鼠24 h食物消耗量和体重，黑白箱实验中白箱停留时间和黑白箱穿梭次数，以及强迫游泳实验不动状态时间和悬尾实验中悬尾不动时间。同时，通过免疫印记法检测三组小鼠海马去NMDA受体（NR2A和NR2B）、cAMP 结合蛋白(CREB) 和钙调蛋白激酶II(CaMKII) 蛋白表达水平。结果：治疗前，产后抑郁小鼠24 h食物消耗量、体重、白箱停留时间、黑白箱穿梭次数以及海马区CREB蛋白表达水平均显著低于对照组小鼠（P<0.05），而游泳不同状态时间、悬尾不动时间和海马区NR2A、NR2B、cAMP蛋白表达水平均显著高于对照组小鼠（P<0.05）。治疗后，针刺百会穴治疗组小鼠24 h食物消耗量、体重、白箱停留时间和黑白箱穿梭次数以及海马区CREB蛋白表达水平均显著高于模型组小鼠（P<0.05），而游泳不同状态时间、悬尾不动时间和海马区NR2A、NR2B、cAMP蛋白表达水平均显著低于模型组小鼠（P<0.05）。结论：针刺百会穴可以显著改善产后抑郁小鼠行为学情况，提高其运动能力，其可能与影响产后抑郁小鼠NMDAR相关蛋白表达有关。

Effects of Acupuncture at Baihui Point on Behavioral Changes and Expression of NMDAR-related Proteins in Hippocampus of Mice with Postpartum Depression

ABSTRACT Objective: To study the effect of acupuncture at Baihui point on the behavioral changes and the expression of N-methyl-D-aspartate receptor (NMDAR)-related proteins in the hippocampus of mice with postpartum depression. Methods: Thirty C57BL/6 female mice were randomly divided into control group, model group and treatment group, 30 mice in each group. The mice in the model group and the treatment group were subcutaneously injected with dexamethasone sodium phosphate to establish a postpartum depression mouse model during pregnancy, and the mice in the control group were subcutaneously injected with the same amount of normal saline as a control. The mice in the treatment group were treated with acupuncture at Baihui point for 28 days after delivery, while the mice in the model group and control group were not treated. The 24-hour food consumption and body weight of the three groups of mice, the time spent in the white box and the number of shuttles in the black and white box in the black and white box experiment, and the immobility time in the forced swimming experiment and the immobility time in the tail suspension experiment were compared. At the same time, the expression levels of NMDA receptors (NR2A and NR2B), cAMP-binding protein (CREB) and calmodulin kinase II (CaMKII) protein in the hippocampus of the three groups of mice were detected by immunoblotting. Results: Before treatment, the 24-hour food consumption, body weight, white box residence time, black and white box shuttle times and CREB protein expression in hippocampus of postpartum depression mice were significantly lower than those of control mice (P<0.05). The time of swimming in different states, the time of tail suspension and the protein expression levels of NR2A, NR2B and cAMP in the hippocampus were significantly higher than those in the control group (P<0.05). After treatment, the 24-hour food consumption, body weight, white box residence time, number of black and white box shuttles, and CREB protein expression in hippocampus of the acupuncture Baihui point treatment group were significantly higher than those of the model group (P<0.05). However, the swimming time in different states, tail suspension immobility time and the expression levels of NR2A, NR2B and cAMP proteins in the hippocampus were significantly lower than those in the model group (P<0.05). Conclusion: Acupuncture at Baihui point can significantly improve the behavior of mice with postpartum depression and improve their exercise ability, which may be related to the expression of NMDAR-related proteins in mice with postpartum depression.