Cyclic AMP mediates heat stress response by the control of redox homeostasis and ubiquitin-proteasome system |
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Authors: | Annalisa Paradiso Guido Domingo Emanuela Blanco Alessio Buscaglia Stefania Fortunato Milena Marsoni Pasquale Scarcia Sofia Caretto Candida Vannini Maria Concetta de Pinto |
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Affiliation: | 1. Department of Biology, University of Bari “Aldo Moro”, Bari, Italy;2. Department of Biotechnology and Life Science, University of Insubria, Varese, Italy;3. Institute of Biosciences and Bioresources, National Research Council, Bari, Italy;4. Department of Biology, University of Bari “Aldo Moro”, Bari, Italy Department of Biotechnology and Life Science, University of Insubria, Varese, Italy;5. Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “Aldo Moro”, Bari, Italy;6. Institute of Sciences of Food Production, CNR, Research Division Lecce, Lecce, Italy |
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Abstract: | Heat stress (HS), causing impairment in several physiological processes, is one of the most damaging environmental cues for plants. To counteract the harmful effects of high temperatures, plants activate complex signalling networks, indicated as HS response (HSR). Expression of heat shock proteins (HSPs) and adjustment of redox homeostasis are crucial events of HSR, required for thermotolerance. By pharmacological approaches, the involvement of cAMP in triggering plant HSR has been recently proposed. In this study, to investigate the role of cAMP in HSR signalling, tobacco BY-2 cells overexpressing the ‘cAMP-sponge’, a genetic tool that reduces intracellular cAMP levels, have been used. in vivo cAMP dampening increased HS susceptibility in a HSPs-independent way. The failure in cAMP elevation during HS caused a high accumulation of reactive oxygen species, due to increased levels of respiratory burst oxidase homolog D, decreased activities of catalase and ascorbate peroxidase, as well as down-accumulation of proteins involved in the control of redox homeostasis. In addition, cAMP deficiency impaired proteasome activity and prevented the accumulation of many proteins of ubiquitin-proteasome system (UPS). By a large-scale proteomic approach together with in silico analyses, these UPS proteins were identified in a specific cAMP-dependent network of HSR. |
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Keywords: | cyclic AMP heat shock proteins heat stress response proteomics reactive oxygen species redox homeostasis tobacco BY-2 cells ubiquitin-proteasome system |
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