基于广泛靶向代谢组学分析黑老虎不同部位成分差异

为提高黑老虎(Kadsura coccinea)资源的综合利用率，采用广泛靶向代谢组学技术鉴定并分析了根、茎、叶代谢组分差异及高度富集成分。结果表明，在根、茎和叶中分别鉴定出642、650和619个代谢物，以酚酸、脂质、类黄酮和有机酸为主；叶与根、茎与根的共有成分分别为566和650个，显著差异成分有442和393个，主要为酚酸、类黄酮和脂质，差异代谢物在苯丙烷生物合成、黄酮与黄酮醇生物合成通路中显著富集。代谢物总丰度和次生代谢物丰度均表现为叶>根>茎，叶中酚酸、类黄酮和脂质及茎中酚酸积累量显著高于根，而氨基酸及其衍生物、萜类、木脂素、香豆素、生物碱的丰度在根中显著上调。因此，黑老虎根、茎、叶有大量共有成分，叶和茎中酚酸、叶中类黄酮和脂质高度富集，含有新绿原酸、绿原酸、槲皮素等多个丰度较高且具有重要生物活性化合物，具有较高利用价值。

Metabolic in Different Tissues of Kadsura coccinea by Using Widely-targeted Metabolomics

To improve the comprehensive utilization of Kadsura coccinea resources, the differential and highly enriched metabolic components of root, stem and leaf were analyzed by using wide range of targeted metabolomics techniques. The results showed that there were 642, 650 and 619 metabolites identified from roots, stems and leaves, respectively, in which phenolic acids, lipids, flavonoids and organic acids were dominant in quantity. There were 566 and 650 common metabolites in leaves and roots, stems and roots, respectively, of which 442 and 393 were significantly different metabolites, mainly including phenolic acids, flavonoids and lipids. Moreover, these differentially metabolites were significantly enriched in pathways of phenylpropane biosynthesis, flavone and flavonol biosynthesis. The total and secondary metabolites abundances were in the order of leaf > root > stem. The accumulation of phenolic acids, flavonoids and lipids in leaves and phenolic acids in stems were significantly higher than those in roots; while the abundance of amino acids and their derivatives, terpenoids, lignans, coumarins and alkaloids were significantly up-regulated in roots. Therefore, there were a large number of common components in roots, stems and leaves of K. coccinea. Phenolic acids, flavonoids and lipids in leaves and stems were highly enriched, and several bioactive compounds, such as neochlorogenic acid, chlorogenic acid and quercetin, were highly abundant and have high utilization value.