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Assessing the potential of environmental DNA metabarcoding for monitoring Neotropical mammals: a case study in the Amazon and Atlantic Forest,Brazil |
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| Authors: | Naiara Guimarães Sales Mariane da Cruz Kaizer Ilaria Coscia Joseph C. Perkins Andrew Highlands Jean P. Boubli William E. Magnusson Maria Nazareth Ferreira Da Silva Chiara Benvenuto Allan D. Mcdevitt |
| Affiliation: | 1. Environment and Ecosystem Research Centre, School of Science, Engineering and Environment, University of Salford, Salford, M5 4WT UK;2. Coordenação de Biodiversidade, Instituto Nacional de Pesquisas da Amazônia, Manaus, Amazonas, Brazil;3. Coleção de Mamíferos, Instituto Nacional de Pesquisas da Amazônia, Manaus, Amazonas, Brazil |
| Abstract: | The application of environmental DNA (eDNA) metabarcoding as a biomonitoring tool has greatly increased, but studies have focused on temperate aquatic macro-organisms. We apply eDNA metabarcoding to detecting the mammalian community in two high-biodiversity regions of Brazil: the Amazon and Atlantic Forests. We identified Critically Endangered and Endangered mammalian species and found overlap with species identified via camera trapping. We highlight the potential for using eDNA monitoring for mammals in biodiverse regions and identify challenges: we need a better understanding of the ecology of eDNA within variable environments and more appropriate reference sequences for species identification in these anthropogenically impacted biomes. |
| Keywords: | Brazil camera traps Critically Endangered eDNA mammals river terrestrial |