Detection by multiplex polymerase chain reaction and typing of Chlamydia trachomatis isolates |
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| Authors: | Marcello Valassina Maria Grazia Cusi Daniele Corsaro Carlo Buffi Giovanna Piazzesi Pier Egisto Valensin |
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| Institution: | Department of Molecular Biology, Microbiology Section, University of Siena, via Laterina 8, 53100 Siena, Italy; Gynecological and Obstetric Department, Burresi Hospital, Poggibonsi (Siena, Italy |
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| Abstract: | Abstract The multiplex polymerase chain reaction (PCR) was applied for the detection of the Chlamydia trachomatis chromosome and plasmid. The multiplex PCR demonstrated a sensitivity of 0.8 fg of chlamydial DNA, corresponding to the detection of about 5 copies of the plasmid. Analysis of 195 genital specimens collected randomly from a female population, showed that the multiplex PCR is more sensitive and rapid than culturing for detecting Chlamydia trachomatis . Moreover, sequencing of the II variable domain of the ompl gene, directly from DNA of the clinical specimens, appears to be a simple and rapid method for determining serovar isolates. |
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| Keywords: | Chlamydia spp Genotype Polymerase chain reaction |
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