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| 利用siRNA高通量测序技术筛查蚊子体内携带的RNA病毒 | |
| 引用本文： | 陈斌,龚正达,张丽云,朱大强,李存,江晓芳,安小平,米志强,范华昊,何后军,陈禹保,童贻刚,刘玮.利用siRNA高通量测序技术筛查蚊子体内携带的RNA病毒[J].生物信息学,2011,9(3):189-191. |
| 作者姓名： | 陈斌 龚正达 张丽云 朱大强 李存 江晓芳 安小平 米志强 范华昊 何后军 陈禹保 童贻刚 刘玮 |
| 作者单位： | 1. 军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京,100071 2. 云南省地方病防治所,大理,671000 3. 江西农业大学动物科技学院,南昌,330045 4. 北京市计算中心计算生物学研究室,北京,100021 |
| 基金项目： | 国家自然科学基金，病原微生物生物安全国家重点实验室课题 |
| 摘 要： | 昆虫可以利用小干扰RNA(siRNA)机制干扰体内RNA病毒的增殖,从而获得对该病毒的免疫能力。通过第二代测序技术对蚊子的小RNA进行高通量测序,再通过生物信息学方法寻找其中的RNA病毒序列,发现在我国云南地区的白纹伊蚊和致倦库蚊体内存在Marayo,Omsk hemorrhagic fever和Ilheus等RNA病毒的基因片段。至此建立了发现媒介昆虫体内携带病毒的一种新方法,可用于媒介昆虫携带RNA病毒的本底调查。 |
| 关 键 词： | 高通量测序 小干扰RNA 病毒 白纹伊蚊 致倦库蚊 |
Screening for RNA Viruses in Mosquitoes by High-throughput Sequencing of siRNA |
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| CHEN Bin,GONG Zheng-da,ZHANG Li-yun,ZHU Da-giang,LI Cun,JIANG Xiao-fang,AN Xiao-ping,MI Zhi-giang,FAN Hua-hao,He Hou-jun,CHEN Yu-bao,TONG Yi-gang,LIU Wei.Screening for RNA Viruses in Mosquitoes by High-throughput Sequencing of siRNA[J].China Journal of Bioinformation,2011,9(3):189-191. | |
| Authors: | CHEN Bin GONG Zheng-da ZHANG Li-yun ZHU Da-giang LI Cun JIANG Xiao-fang AN Xiao-ping MI Zhi-giang FAN Hua-hao He Hou-jun CHEN Yu-bao TONG Yi-gang LIU Wei |
| Institution: | CHEN Bin~1,GONG Zheng-da~2,ZHANG Li-yun~2,ZHU Da-qiang~1,LI Cun~1,JIANG Xiao-fang~1,AN Xiao-ping~1,MI Zhi-qiang~1,FAN Hua-hao~1,He Hou-jun~3,CHEN Yu-bao~4,TONG Yi-gang~1*,LIU Wei~1*(1.State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology,Academy of Military Medical Science,Beijing 100071,China,2.Yunnan Provincial Institute of Endemic Diseases Control and Prevention,Dali 671000,3.College of Animal Science & Technology,Jiangxi agricultural university,Nanchang 330045... |
| Abstract: | Insects obtains immunity against RNA viruses through small interference RNA(siRNA) mechanism.Here we adopt Next-generation sequencing technique to perform high-throughput sequencing of mosquito siRNA and then screening for RNA virus sequences with bioinformatics methods.We found that the Aedes albopictus and Culex quinquefasciatus captured in Yunnan,China contains the genonomic information of Marayo virus,Omsk hemorrhagic fever virus and Ilheus virus.This research established a new strategy to find naturall... |
| Keywords: | high-throughput sequencing small interference RNA virus Aedes albopictus Culex quinquefasciatus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |