| 利用VBA查找核酸数据库DNA保守序列 |
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| 引用本文： | 詹少华,尹艺林,蔡永萍,樊洪泓,林毅.利用VBA查找核酸数据库DNA保守序列[J].生物信息学,2011,9(4):299-302. |
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| 作者姓名： | 詹少华 尹艺林 蔡永萍 樊洪泓 林毅 |
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| 作者单位： | 1. 皖西学院生物与制药工程学院,六安,237012
2. 安徽农业大学生命科学学院,合肥,230036 |
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| 基金项目： | 安徽高校省级自然科学研究重点项目资助 |
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| 摘 要： | 采用VBA编写了查找核酸数据库保守序列的四个相关程序,“导入DNA序列”程序可以将Fasta格式的DNA序列文本文件存放到Excel Sheetl的A列中,保留每个序列的Gi号,删除多余的注释部分；“整理DNA序列”程序可以将DNA序列Gi号存放到A列中,B列为对应Gi号的完整序列；“DNA随机序列”程序可以产生DNA随机序列；“发现DNA保守序列”程序可以将随机序列与下载的DNA序列比对,查找每一种随机序列的出现频率.以大豆基因组序列为实例,说明了这些程序的应用方法.该程序弥补了流行序列比对软件的不足,为PCR设计引物、分析基因功能以及种质资源鉴定等方面提供新的工具.
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| 关 键 词： | VBA 序列比对 保守序列 核酸数据库 大豆 |
Searching conservative sequences in nuclear acid database by VBA programs |
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| ZHAN Shao-Hua,YIN Yi-lin,CAI Yong-Ping,FAN Hong-Hong,LIN Yi.Searching conservative sequences in nuclear acid database by VBA programs[J].China Journal of Bioinformation,2011,9(4):299-302. |
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| Authors: | ZHAN Shao-Hua YIN Yi-lin CAI Yong-Ping FAN Hong-Hong LIN Yi |
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| Institution: | ZHAN Shao-Hua1,YIN Yi-lin1,CAI Yong-Ping2,FAN Hong-Hong2,LIN Yi2 (1.Biological and Pharmacological Engineering Department,West Anhui University,Lu'an,Anhui 237012,China,2.Life Science School,Anhui Agricultural University,Hefei Anhui 230036,China) |
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| Abstract: | The four VBA (visual basic for application) programs were written for searching conservative sequences in nuclear acid database.The programs included importing-DNA-sequence,sorting-DNA-sequence,DNA-random-sequence and finding-DNA-conservative-sequence.The DNA sequences saved as fasta format in text file could be imported into column A of Excel Sheet1 by the program of importing-DNA-sequence,at same time,the Gi numbers were reserved and the redundant notes were deleted.Then,the Gi numbers were sorted into co... |
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| Keywords: | Visual Basic for Application (VBA) Sequence alignment Conservative sequence Nuclear acid database Soybean |
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