| 黑曲霉果胶裂解酶的生物信息分析 |
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| 引用本文： | 柯崇榕,杨欣伟,林晓华,吴毕莎,陈荣珠,黄建忠.黑曲霉果胶裂解酶的生物信息分析[J].生物信息学,2011,9(3):192-195. |
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| 作者姓名： | 柯崇榕 杨欣伟 林晓华 吴毕莎 陈荣珠 黄建忠 |
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| 作者单位： | 福建师范大学工业微生物教育部工程研究中心,生命科学学院,福建省现代发酵技术工程研究中心,福建,福州,350108 |
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| 摘 要： | 用生物信息方法对果胶裂解酶(PNL)基因的核酸序列及其推导氨基酸序列的组成、亚细胞定位、疏水性/亲水性以及二、三级结构等进行分析.结果表明,黑曲霉的PNL为具有一定亲水性的稳定酸性分泌蛋白,具有明显的信号肤,无跨膜结构区,保守功能结构域为Pee_lyase_C.二级结构主要构成是不规则卷曲,具有以β片层结构为基础的相似三维空间结构.
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| 关 键 词： | 黑曲霉 果胶裂解酶 pel 生物信息学 |
Bioinformatics Analysis of pectin lyases in Aspergillus niger |
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| KE Chong-rong,YANG Xin-wei,LIN Xiao-hua,WU Bi-sha,CHEN Rong-zhu,HUANG Jian-zhong.Bioinformatics Analysis of pectin lyases in Aspergillus niger[J].China Journal of Bioinformation,2011,9(3):192-195. |
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| Authors: | KE Chong-rong YANG Xin-wei LIN Xiao-hua WU Bi-sha CHEN Rong-zhu HUANG Jian-zhong |
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| Affiliation: | KE Chong-rong,YANG Xin-wei,LIN Xiao-hua,WU Bi-sha,CHEN Rong-zhu,HUANG Jian-zhong*(Engineering Research Center of Industrial Microbiology,Ministry of Education,College of Life Sciences,Engineering Research Center of Fujian Modern Fermentation Technology,Fujian Normal University,Fuzhou 350108,China) |
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| Abstract: | Analyzed and predicted the nucleotide and amino acids composition, sub -cell location, signal peptides and tran- membrane regions ,hydrophobicity/hydrophilicity,secondary and tertiary structure of pectin lyases inclu- ding Aspergillus niger from NCBI database. Results shows that A. niger PNLs are stability of hydrophilic secreted protein, with a clear signal peptide, non - transmembrane area. The structures of PNL include a characteristic functional domain of Pec_lyase_C. Random coil is the main components of its secondary structure and has similar tertiary structures with the β sheet structure. |
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| Keywords: | Aspergillus niger pectin lyases PNL bioinformatics |
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