| 抗HIV融合多肽CP32M的制备工艺研究 |
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| 引用本文： | 付超,郭会芹,王孝花,胡洁,戴秋云.抗HIV融合多肽CP32M的制备工艺研究[J].生物技术通讯,2011,22(6):859-863. |
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| 作者姓名： | 付超 郭会芹 王孝花 胡洁 戴秋云 |
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| 作者单位： | 军事医学科学院生物工程研究所,北京,100071 |
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| 基金项目： | 新药创制重大专项，国家自然科学基金 |
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| 摘 要： | 目的:研究抗HIV融合多肽CP32M的制备工艺,为其临床前试验奠定基础。方法:采用固-液相混合策略规模合成目标肽,用离子交换色谱、反相高效液相色谱对粗肽进行纯化。结果:获得了利于提高合成及片段缩合效率的3条优选片段,CP32M粗品经DEAE离子交换色谱及C18反相纯化后纯度高于98%。结论:CP32M按3条片段(Ac-1-9-OH、Fmoc-10-21-OH、H-22-32-NH2)划分,可显著提高片段合成及缩合效率,DEAE阴离子交换色谱能除去大部分杂质,提高了第二步C18反相色谱纯化效率。
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| 关 键 词： | 抗HIV融合多肽 CP32M 多肽合成 固液相混合策略 |
Preparation Method of Anti-HIV Fusion Peptide CP32M |
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| FU Chao,GUO Hui-Qin,WANG Xiao-Hua,HU lie,DAI Qiu-Yun.Preparation Method of Anti-HIV Fusion Peptide CP32M[J].Letters in Biotechnology,2011,22(6):859-863. |
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| Authors: | FU Chao GUO Hui-Qin WANG Xiao-Hua HU lie DAI Qiu-Yun |
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| Institution: | FU Chao,GUO Hui-Qin,WANG Xiao-Hua,HU Jie,DAI Qiu-Yun Beijing Institute of Biotechnology,Beijing 100071,China |
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| Abstract: | Objective: In order to provide a large quantity of CP32M,an anti-HIV-1 fusion peptide,for its preclinical trial,we probe its large scale of synthesis.Methods: CP32M was synthesized by using combined solid-liquid method,the crude peptide was purified by DEAE anion exchange chromatography and RP-HPLC.Results: Three optimized fragments which were easily synthesized and coupled were found,CP32M with purity more than 98% was obtained after the purification of DEAE anion exchange chromatography and RP-HPLC.Conclu... |
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| Keywords: | anti-HIV fusion peptide CP32M peptide synthesis solid-liquid phase combined strategy |
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