| 蕨菜黄酮分离鉴定 |
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| 引用本文： | 陈乃富,陈乃东,陈存武,张莉,胡先敏.蕨菜黄酮分离鉴定[J].生物学杂志,2011,28(6):87-89. |
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| 作者姓名： | 陈乃富 陈乃东 陈存武 张莉 胡先敏 |
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| 作者单位： | 1. 皖西学院生物与制药工程学院,安徽六安,237012;植物细胞工程安徽省工程技术研究中心,安徽六安,237012
2. 皖西学院生物与制药工程学院,安徽六安,237012 |
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| 基金项目： | 安徽省自然科学基金，安徽省高校省级自然科学研究重点项目，安徽省高校省级自然科学研究一般项目 |
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| 摘 要： | 采用75%的乙醇提取经乙醚脱脂的蕨菜粉末,提取液回收乙醇后以石油醚、氯仿、乙酸乙酯、正丁醇依次萃取粗分段,硅胶柱层析、乙酸乙酯-甲醇系统对乙酸乙酯部位梯度洗脱,对其乙酸乙酯洗脱部分反复凝胶柱层析获得2个黄酮单体,分别鉴定为紫云英苷(1)、芦丁(2),其中化合物1为首次从蕨菜中分离得到。
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| 关 键 词： | 蕨菜 紫云英苷 芦丁 |
Isolation and Identification of Flavonoids from Pteridium aquilinum(L.)Kuhn |
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| CHEN Nai-fu,CHEN Nai-dong,CHEN Cun-wu,Zhang Li,HU Xian-min.Isolation and Identification of Flavonoids from Pteridium aquilinum(L.)Kuhn[J].Journal of Biology,2011,28(6):87-89. |
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| Authors: | CHEN Nai-fu CHEN Nai-dong CHEN Cun-wu Zhang Li HU Xian-min |
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| Institution: | CHEN Nai-fu1,2,CHEN Nai-dong1,CHEN Cun-wu1,Zhang Li1,HU Xian-min1(1.College of Biotechnology and Pharmaceutical Engineering,West Anhui University,Lu'an 237012,2.Anhui Biotechnology Research Center of Plant Cell Engineering,China) |
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| Abstract: | The power of Pteridium aquilinum（L.） Kuhn degreased by ethylether of P. aquilinum was extracted by 75% aqueous ethanol three times at 80℃. The concentrated dark-brown residue was suspended in small excess of water and then partitioned by petroleum ether, chloroform, acetoacetate, n-butanol, separately. The extract of the acetoacetate was chromatographied on silica gel with acetoacetate-methyl gradiently. The acetoacetate part was then successfully subjected to a Sephadex LH 20 column with aqueous MeOH and yielded two flavonoids （1,2）. The structures were elucided as kaempferol-3-O-β-D-glucopyranoside（=astragalin,1）, which was isolated from P. aquilinum for the first time, and rutin（2）on the basis of their spectrometry data. |
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| Keywords: | Pteridium aquilinum kaempferol-3-O-β-D-glucopyranoside（=astragalin） rutin |
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