|
|||||
|
|
| 一种简便的适用于酵母双杂交系统的酵母质粒提取方法 | |
| 引用本文： | 苑丽娜,王定和,王永杰,郭佳,单卫星.一种简便的适用于酵母双杂交系统的酵母质粒提取方法[J].生物技术通讯,2011,22(6):864-866. |
| 作者姓名： | 苑丽娜 王定和 王永杰 郭佳 单卫星 |
| 作者单位： | 1. 西北农林科技大学生命科学学院,陕西杨凌,712100 2. 西北农林科技大学植物保护学院,陕西杨凌,712100 3. 河南省郸城县第一高级中学,河南郸城,477150 4. 西北农林科技大学植物保护学院,陕西杨凌712100;陕西省农业分子生物学重点实验室,陕西杨凌712100 |
| 基金项目： | 高等学校学科创新引智计划，现代农业产业技术体系 |
| 摘 要： | 目的:建立一种适用于酵母双杂交系统的简便快捷的酵母质粒提取方法。方法:以酿酒酵母为供试材料,用玻璃珠振荡法破除酵母细胞壁,提取酵母总DNA,最后通过电转化大肠杆菌DH10B获得目的质粒。结果:粗提得到的质粒可直接转化DH10B,作为模板用于PCR分析及酵母双杂交后续的序列分析等,大大降低了工作量。结论:该方法简便快捷,经济实用,降低了成本,提高了效率,可以作为一种实验室酵母质粒提取方法。 |
| 关 键 词： | 玻璃珠法质粒提取 酵母质粒 酵母双杂交 |
A Simple Method for Isolation of Yeast Plasmid Used for Yeast Two-Hybrid System |
|
| YUAN Li-Na,WANG Ding-He,WANG Yong-Jie,GUO Jia,SHAN Wei-Xing.A Simple Method for Isolation of Yeast Plasmid Used for Yeast Two-Hybrid System[J].Letters in Biotechnology,2011,22(6):864-866. | |
| Authors: | YUAN Li-Na WANG Ding-He WANG Yong-Jie GUO Jia SHAN Wei-Xing |
| Institution: | YUAN Li-Na1a,WANG Ding-He1b,WANG Yong-Jie3,GUO Jia1b,SHAN Wei-Xing1b,2 1.a.College of Life Science,b.College of Plant Protection,Northwest A&F University,Yangling 712100,2.Shaanxi Key Laboratory of Molecular Biology for Agriculture,3.No.1 Middle School of Dancheng,Dancheng,Henan 477150,China |
| Abstract: | Objective: To establish a fast,stable and economical isolation method of plasmid from yeast for the yeast two-hybrid,a glass beads-based simple method for isolation of plasmid DNA from yeast Saccharomyces cerevisiae was developed.Methods: Yeast cells were mixed with glass beads and the target plasmids released by vortexing,preceipitated and transformed into E.coli DH10B by electroporation.Results: Agarose gel electrophoresis showed that the quality of the extracted plasmid in the crude yeast plasmid DNA was... |
| Keywords: | glass beads-based plasmid extaction yeast plasmid yeast two-hybrid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |