| 弗氏2a志贺菌htrA基因突变体的构建 |
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| 引用本文： | 马姗姗,朱力,古月华,刘先凯,冯尔玲,王恒樑.弗氏2a志贺菌htrA基因突变体的构建[J].生物技术通讯,2011,22(6):781-784. |
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| 作者姓名： | 马姗姗 朱力 古月华 刘先凯 冯尔玲 王恒樑 |
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| 作者单位： | 军事医学科学院生物工程研究所,病原微生物生物安全国家重点实验室,北京100071 |
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| 摘 要： | 目的:构建弗氏2a志贺菌2457T的htrA基因缺失突变株及HtrA酶失活突变株,以便进一步研究HtrA蛋白的功能。方法:用PCR扩增htrA基因上下游同源臂,构建含有kan基因的打靶片段,采用λ-Red重组系统对htrA基因进行缺失,用PCR进行验证;通过定点突变的方法构建HtrA酶失活突变株,并测序验证。结果与结论:构建了2457T htrA缺失突变株和2457T/htrAFSA酶失活突变株。
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| 关 键 词： | 弗氏2a志贺菌2457T株 HtrA蛋白 缺失突变体 定点突变 |
Construction of HtrA Mutants of Shigella flexneri |
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| MA Shan-Shan,ZHU Li,GU Yue-Hua,LIU Xian-Kai,FENG Er-Ling,WANG Heng-Liang.Construction of HtrA Mutants of Shigella flexneri[J].Letters in Biotechnology,2011,22(6):781-784. |
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| Authors: | MA Shan-Shan ZHU Li GU Yue-Hua LIU Xian-Kai FENG Er-Ling WANG Heng-Liang |
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| Institution: | MA Shan-Shan,ZHU Li,GU Yue-Hua,LIU Xian-Kai,FENG Er-Ling,WANG Heng-Liang Beijing Institute of Biotechnology,State Key Laboratory of Pathogen and Biosecurity,Beijing 100071,China |
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| Abstract: | Objective： To explore the function of htrA gene by constructing an htrA deletion mutant and an HtrA enzyme-inactived mutant of Shigella flexneri 2a strain 2457T.Methods： htrA gene was deleted by using λ-Red recombination system,and confirmed by PCR.The HtrA enzyme-inactived mutant was constructed by using site-directed mutagenesis method,and confirmed by sequencing.Results Conclusion： The htrA deletion mutant and HtrA enzyme-inactived mutant of S.flexneri 2a strain 2457T were constructed. |
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| Keywords: | Shigella flexneri 2a strain 2457T HtrA deletion mutant site-directed mutagenesis |
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