|
|||||
|
|
| 人工microRNA抗乙型肝炎病毒效果初探 | |
| 引用本文： | 赵海峰,李丹丹,李英,胡伟,王学军,王升启.人工microRNA抗乙型肝炎病毒效果初探[J].生物技术通讯,2011,22(6):769-772. |
| 作者姓名： | 赵海峰 李丹丹 李英 胡伟 王学军 王升启 |
| 作者单位： | 1. 军事医学科学院放射与辐射医学研究所,北京100850;天津中医药大学,天津300193 2. 军事医学科学院放射与辐射医学研究所,北京100850;安徽医科大学,安徽合肥230032 3. 军事医学科学院放射与辐射医学研究所,北京100850; |
| 基金项目： | 国家传染病防治科技重大专项，国家杰出青年基金 |
| 摘 要： | 目的:设计靶向乙型肝炎病毒(HBV)基因保守区的人工microRNA(amiRNA),考察其对HBV基因表达的抑制作用。方法:比对HBV全基因组现有序列,选择保守区设计amiRNA,定向克隆到pcDNA6.2-GW/EmGFP-miR载体,将amiRNA载体与HBV复制载体pHBV1.31共转染HepG2细胞,72 h后收取细胞上清,ELISA检测HBV表面抗原(HBsAg)及e抗原(HBeAg)的含量,荧光定量PCR检测HBV DNA含量。结果:amiRNA可显著抑制细胞上清HBsAg、HBeAg和HBV DNA的水平。结论:amiRNA作为防治HBV感染的潜在有效手段之一值得进一步深入研究。 |
| 关 键 词： | 乙型肝炎病毒 人工microRNA RNA干扰 |
Artificial microRNA Can Suppress Hepatitis B Virus Replication Efficiently In Vitro |
|
| ZHAO Hai-Feng,LI Dan-Dan,LI Ying,HU Wei,WANG Xue-Jun,WANG Sheng-Qi.Artificial microRNA Can Suppress Hepatitis B Virus Replication Efficiently In Vitro[J].Letters in Biotechnology,2011,22(6):769-772. | |
| Authors: | ZHAO Hai-Feng LI Dan-Dan LI Ying HU Wei WANG Xue-Jun WANG Sheng-Qi |
| Institution: | ZHAO Hai-Feng1,2,LI Dan-Dan1,3,LI Ying1,HU Wei1,WANG Xue-Jun1,WANG Sheng-Qi1 1.Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,2.Tianjin University of Traditional Chinese Medicine,Tianjin 300193,3.Anhui Medical University,Hefei 230032,China |
| Abstract: | Objective: To screen artificial microRNA(amiRNA) which can inhibit hepatitis B virus(HBV) replication in vitro.Methods: amiRNA targeted HBV conserved regions were designed and cloned into the vector pcDNA6.2-GW/EmGFP-miR.The inhibition rate of different amiRNA were compared by cotransfection amiRNA vectors and the HBV replicon vector pHBV1.31 into HepG2 cells.After 72 h,HBsAg,HBeAg and HBV DNA were assayed and analyzed.Results: amiRNA can suppress HBV replication efficiently in vitro.Conclusion: amiRNA as a... |
| Keywords: | hepatitis B virus artificial microRNA RNA interference |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |