|
|||||
|
|
| 用SDS-PAGE和Vorum银染法比较真菌菌丝体破壁效果 | |
| 引用本文： | 李敏惠,潘克俭,宋丽萍,钟文英,邹珂珂,杨平.用SDS-PAGE和Vorum银染法比较真菌菌丝体破壁效果[J].生物技术通讯,2011,22(6):867-869. |
| 作者姓名： | 李敏惠 潘克俭 宋丽萍 钟文英 邹珂珂 杨平 |
| 作者单位： | 1. 成都医学院科研实验中心,四川成都,610083 2. 成都医学院生物医学系,四川成都,610083 3. 成都医学院生物化学教研室,四川成都,610083 |
| 基金项目： | 四川省教育厅青年基金，成都医学院创新性实验项目 |
| 摘 要： | 目的:研究不同处理方法对丝状真菌Pythium sp.GY1938菌丝体破壁效果的差异。方法:采用7种方法对真菌菌丝体进行破壁处理,将制备的胞内可溶蛋白质样品通过SDS-PAGE和Vorum法银染处理,比较不同破壁方法处理后蛋白质释放效果的优劣。结果:方法VI(石英砂、液氮、研磨)的破壁效果最好,蛋白质的分离效率和提取质量最高。结论:方法VI适合于丝状真菌Pythium sp.GY1938蛋白组学研究中目的蛋白的提取。 |
| 关 键 词： | 丝状真菌 Pythium sp.GY1938 破壁 银染 |
Comparison of the Effect for Filamentous Fungal Breaking-Wall by SDS-PAGE and Vorum Silver Staining |
|
| LI Min-Hui,PAN Ke-Jian,SONG Li-Ping,ZHONG Wen-Ying,ZOU Ke-Ke,YANG Ping.Comparison of the Effect for Filamentous Fungal Breaking-Wall by SDS-PAGE and Vorum Silver Staining[J].Letters in Biotechnology,2011,22(6):867-869. | |
| Authors: | LI Min-Hui PAN Ke-Jian SONG Li-Ping ZHONG Wen-Ying ZOU Ke-Ke YANG Ping |
| Institution: | LI Min-Huia,PAN Ke-Jianb,SONG Li-Pingb,ZHONG Wen-Yingb,ZOU Ke-Keb,YANG Pingc a.Center of Science and Research,b.Department of Biomedicine,c.Department of Biochemistry,Chengdu Medical College,Chengdu 610083,China |
| Abstract: | Objective: To compare the effects in the release of the intracellular protein in Pythium sp.GY1938 strain.Methods: Seven kinds of treatment including grinding with silica sand,sonication after grinding with silica sand,sonication with silica sand,sonication,grinding with liquid nitrogen,grinding with silica sand and liquid nitrogen,sonication after grinding with silica sand and liquid nitrogen were used in this research.Then,each lysate was treated with SDS-PAGE and the efficiency was evaluated by Vorum sil... |
| Keywords: | filamentous fungi Pythium sp GY1938 breaking-wall silver staining |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |