Effect of Low Temperature on the Protein Metabolism of Wheat Leaves |
| |
Authors: | M.L. Pinedo G.F. Hernández R.D. Conde J.A. Tognetti |
| |
Affiliation: | (1) Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina e-mail;(2) Cátedra de Fisiología Vegetal, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, CC 276, 7620 Balcarce, Argentina |
| |
Abstract: | The effect of low temperature on the protein metabolism of wheat primary leaves was examined. In seedlings transferred from 25 to 5 °C, total soluble protein accumulation, in vivo protein synthesis and breakdown, in vitro protein breakdown, and SDS-PAGE profiles of proteinases in gelatine-containing gels were analysed. Leaf protein content increased within a 7-d period (70 % over the initial value) in plants exposed to 5 °C. The fast protein accumulation observed on days 0 – 2 was mainly attributed to a decreased breakdown. In further days, parallelly to a slowdown in the rate of protein accumulation, the leaf proteolytic activity increased. The incubation temperature also had an influence on the proteolytic activity: Q10 values for the 15 – 5 °C range were 80 – 200 % higher than those observed for the 25 – 15 °C range. On the other hand, the in vivo protein synthesis capacity, at either 25 or 55 °C, was not significantly modified in cold-treated plants. In addition to the enhanced activities of two serine-proteinases (previously found in control plants by SDS-PAGE analysis), cold-treated plants displayed a new proteinase, which had not been detected so far. |
| |
Keywords: | chilling proteases protein synthesis protein degradation Triticum aestivum L. |
本文献已被 SpringerLink 等数据库收录! |
|