Improvement of the transformation efficiency of Flammulina velutipes Fv-1 using the glyceraldehyde-3-phosphate dehydrogenase gene promoter |
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| Authors: | Maehara Tomoko Tomita Shizuko Takabatake Koji Kaneko Satoshi |
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| Institution: | Food Biotechnology Division, National Food Research Institute, Tsukuba, Ibaraki, Japan. |
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| Abstract: | To improve the expression level of heterologous genes in Flammulina velutipes Fv-1, we constructed new vectors having glyceraldehydes-3-phosphate dehydrogenase (gpd) gene promoter to control the expression of target genes. When the hygromycin B phosphotransferase (hph) gene from Escherichia coli was controlled by the gpd promoter, transformation efficiency was 3-fold higher than the case of that controlled by the tryptophan synthetase gene (trp1) promoter. |
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