Isolation and Properties of Deoxyribonucleic Acid from Protoplasts of Cell Suspension Cultures of Ammi visnaga and Carrot (Daucus carota) |
| |
| Authors: | Ohyama K Gamborg O L Miller R A |
| |
| Affiliation: | Prairie Regional Laboratory, National Research Council of Canada, Saskatoon, Saskatchewan S7N OW9, Canada. |
| |
| Abstract: | A procedure is described for the isolation of native DNA from protoplasts of ammi (Ammi visnaga) and carrot (Daucus carota) cells. Protoplasts were produced from 40 grams of fresh cells by enzyme hydrolysis and lysed with sodium dodecyl sulfate. The DNA was purified by treatment with pronase and ribonuclease. Final isolation was achieved by sucrose density gradient centrifugation. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
