生物传感器快速测定BOD系统的稳定性研究

在前期研究工作的基础上，确定了生物传感器快速测定BOD的实验流程。研究了温度对检测结果的影响，初始BOD浓度与检测时间的关系，并探讨了检测结果的重现性及其线性范围。结果表明：恒温环境可以保持微生物活性的相对稳定，并保证了检测过程的相对稳定，检测结果的最大相对偏差7.7%，满足BOD快速测定的要求；溶液BOD浓度不同，所需的检测时间也不同，BOD浓度越高，所需时间越长。在曝气状态下，可以保证BOD浓度在200mg/l以下的样品在15min以内检测完毕。通过对线性范围的分析，该方法适宜的线性范围可达5－200mg/l。

The stability of rapid BOD analysis by biosensor

Based on the previous research results, a flow chart for BOD rapid analysis was determined. The effects of temperature on the stability of measurement result and the initial BOD concentration on measurement time were investigated. The reproducibility of the results and the linear range were also studied. The experimental results indicated that constant temperature could keep the initial DO and the microbial activity relatively stable and guarantee the stability of measurement. The maximal relative error was less than 7.7%, which meet the needs of BOD rapid analysis. It took different time for determining samples containing different BOD values, however, it took less than 15 min when at the initial BOD value below 200mg/L. The linear range for BOD rapid measurement using this system was between 5~200 mg/L.