Characterization of seed nuclei in glucagon aggregation using light scattering methods and field-flow fractionation |
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Authors: | Cindy C Hoppe Lida T Nguyen Lee E Kirsch John M Wiencek |
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Affiliation: | (1) Department of Chemical & Biochemical Engineering, University of Iowa, 52242 Iowa City, IA, USA;(2) Division of Pharmaceutics, University of Iowa, 52242 Iowa City, IA, USA;(3) Department of Chemical Engineering, University of South Florida, 33520 Tampa, FL, USA |
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Abstract: | Background Glucagon is a peptide hormone with many uses as a therapeutic agent, including the emergency treatment of hypoglycemia. Physical instability of glucagon in solution leads to problems with the manufacture, formulation, and delivery of this pharmaceutical product. Glucagon has been shown to aggregate and form fibrils and gels in vitro. Small oligomeric precursors serve to initiate and nucleate the aggregation process. In this study, these initial aggregates, or seed nuclei, are characterized in bulk solution using light scattering methods and field-flow fractionation. |
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