视黄醇结合蛋白4在非酒精性脂肪肝病大鼠组织中的表达

目的：视黄醇结合蛋白4（RBP4）在非酒精性肝脂肪变模型大鼠中的表达情况,以探求其在疾病发生、发展中的意义。方法：40只wistar大鼠随机分为对照组和造模组,分析各组在2、4、6、8周4个时间点血清ALT、AST、TG、TC的变化及肝组织RBP4的表达情况。结果：随着造模时间延长,造模组大鼠肝脏脂肪变越来越明显,血清ALT、AST、TG、TC逐渐升高（p〈0.05）。造模组肝组织RBP4的mRNA的表达随造模时间逐渐增强;造模组免疫组化结果发现,RBP4的表达随造模时间逐渐增强（p〈0.05）。结论：在大鼠非酒精性脂肪肝模型中,RBP4的表达随造模时间延长而增加,与同期对照组相比有统计学差异,因此RBP4可能作为一个敏感的指标反映非酒精性脂肪肝的发生及发展情况。

Expression of Retinol Binding Protein 4 in Non-alcoholic Fatty Liver Tissue of Rats

Objective：To research the influence of Retinol-Binding Protein 4（RBP4） on the pathogenesis and the development of non-alcoholic fatty liver disease（NAFLD） by studying expression of RBP4 in NAFLD model in rats.Method：A total of 40 Wistar rats were randomly divided into two groups：the control group and the NAFLD model group.The levels of ALT,AST,TG,TC in serum together with the RBP4 expression in hepatic tissue were recorded and analyzed on two,four,six and eight weeks postoperation,respectively.Results：The model group rat liver fatty change is more and more obvious as time going.The levels of ALT,AST,TG,TC in model group rose gradually（p0.05）.The mRNA expression of RBP4 in model group is significantly stronger than that in the control group（p0.05）.The immunohistochemical expression of RBP4 in model group is significantly stronger than that in the control group（p0.05）.Conclusion：RBP4 expresses on hepatic cells in early stage of adipose degeneration in NAFLD rat model and increases with time.Thus,RBP4 may be used as a sensitive indicator for the pathogenesis and the development of NAFLD,which can assist in the early diagnosis of the disease.