甲基苯丙胺中毒大鼠相关脑区Caspase-3和Bcl-2表达的研究

目的：研究凋亡相关因子Caspase-3和Bcl-2在甲基苯丙胺神经毒性机制中所发挥的作用。方法：40只健康雄性SD大鼠随机分为对照组（n=10）和实验组（又分为3个亚组,分别为注射1天后、4天后、7天后,n=10）。实验组给予20mg/kg的甲基苯丙胺腹腔注射,对照组给予相同剂量的生理盐水注射。用免疫组化检测中毒大鼠相关脑区Caspase-3和Bcl-2的表达,用图像分析技术对检测结果进行分析。结果：Caspase-3在中毒大鼠不同脑区表达逐渐增加并可见明显的阳性信号;Bcl-2在中毒大鼠不同脑区表达逐渐减弱。结论：凋亡相关因子Caspase-3和Bcl-2参与了甲基苯丙胺神经毒性机制。

Expressions of Caspase-3 and Bcl-2 in Related Encephalic Region of Rats with Poisoning of Methamphetamine

Objective：To study the function of Caspase-3 and Bcl-2 protein in the methamphetamine mechanism.Methods：Forty male Sprague-Dawley rats were randomly divided into control group（n=10） and experimental group（dividing it into the first,fourth and seventh group after the injection,n=10）.Rats in the experimental group were intraperitoneal injected with methamphetamine hydrochlo-ride（20mg/kg）,and those in the control group were injected with saline with the same volume.Then examining the protein expression of Caspase-3 and Bcl-2 of poisoning rats in related encephalic region by immunohistochemistry technique and analyzing the test results through image.Results：Expression of Caspase-3 increased significantly in the related encephalicregion in rat poisoning of metham-phetamine where exist more clearly positive signal.Express of Bcl-2 the reduced gruadually in the related encephalicregion in rat poison-ing of methamphetamine.Conclusion：The abnormal expression of Caspase-3 and Bcl-2 shows that they take part in the Metham-phetamine-Induced Neurotoxicity.