氧自由基与脑胶质瘤瘤周水肿关系的实验研究

目的：探讨自由基和脑胶质瘤瘤周水肿的关系。方法：体外培养大鼠C6脑胶质瘤细胞株,采用立体定向技术将细胞株接种在右侧尾状核,建立大鼠脑胶质瘤模型,共60只,术后5天将荷瘤大鼠随机分为3组（EDA高剂量组,EDA低剂量组和对照组）,每组各20只,每组10只用来测定荷瘤大鼠瘤周脑组织含水量、SOD活性及MDA含量,剩余10只用来观察荷瘤大鼠生存时间。结果：EDA干预组荷瘤大鼠瘤周脑组织含水量下降,SOD活性增高,MDA含量下降,以EDA高剂量组更为显著。各组荷瘤大鼠瘤周脑组织含水量与SOD活性呈负相关,而与MDA含量呈正相关。且EDA组荷瘤大鼠生存时间延长。结论：由基参与大鼠脑胶质瘤瘤周水肿的形成,自由基清除剂能够减轻大鼠脑胶质瘤瘤周脑组织水肿。

Relationship Between Oxygen Free Radical and Peritumor Edema in Glioma

Objective：To investigate the relationship between oxygen free radical and peritumor edema in glioma.Methods：The rat C6 glioma cells were cultivated in vitro.The suspention of C6 glioma cells was stereotaxically injected into right caudate to establish rat C6 glioma model.After surgery,all rat glioma models were randomly classified into 3 groups（edaravone-trteated group in high dose,edaravone-trteated group in low dose and control group）.Each group had twenty rats.Ten rats from each group were used to determine the moisture content in peritumor brain tissues,SOD activity and the content of MDA.The other 10 rats were used to detect the survival time after surgery.Results：The decreased moisture and MDA contents in peritumor brain tissues and increased SOD activity were found in edaravone-treated group,especially in high dose.The survival time was longer in edaravone-treated group than that of control group.Conclusion：Oxygen free radical is involved in the formation of peritumor edema in glioma,and the free radical scavenger may reduce the peritumor edema in glioma.