| 不同加热方式对嗜肺军团菌环介导等温扩增检测法的影响 |
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| 引用本文： | 吕沁风,吴忠华,郑伟,徐琦,孟军,李禾. 不同加热方式对嗜肺军团菌环介导等温扩增检测法的影响[J]. 生物磁学, 2011, 0(3): 493-496,501 |
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| 作者姓名： | 吕沁风 吴忠华 郑伟 徐琦 孟军 李禾 |
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| 作者单位： | 浙江国际旅行卫生保健中心,江苏杭州310003 |
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| 基金项目： | 浙江出入境检验检疫局科技计划项目（ZK2008012） |
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| 摘 要： | 目的：研究不同的加热方式对嗜肺军团菌环介导等温扩增检测法的影响方法：用已知的13株嗜肺军团菌样本,采用空气浴、水浴和PCR仪同时进行环介导等温扩增,观察沉淀反应、荧光反应以及产物电泳结果。结果：水浴和PCR仪加热LAMP反应的沉淀产物较多,荧光反应较强,电泳检测结果较为明显。空气浴的3种检测结果均较弱。结论：采用水浴和PCR仪进行环介导等温扩增反应的效果较好,从仪器设备的成本及实验条件考虑,采用水浴是环介导等温扩增反应首选的加热方式。
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| 关 键 词： | 环介导等温检测 加热方式 嗜肺军团菌 水浴 |
Effect of Different Heating Methods on Legionella Pneumophila Loop-mediated Isothermal Amplification Detection |
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| LU Qing-feng,WU Zhong-hua,ZHENG Wei,XU Qi,MENG Jun,LI He. Effect of Different Heating Methods on Legionella Pneumophila Loop-mediated Isothermal Amplification Detection[J]. Biomagnetism, 2011, 0(3): 493-496,501 |
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| Authors: | LU Qing-feng WU Zhong-hua ZHENG Wei XU Qi MENG Jun LI He |
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| Affiliation: | (Zhejiang international travel health centre,Hangzhou,Jiangsu 310003,China) |
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| Abstract: | Objective：To investigate the effect of the three heating methods on loop-mediated isothermal amplification（LAMP） detection of Legionella pneumophila..Methods：DNA of 13 strains of Legionella pneumophila were amplified by LAMP based on air bath,water bath,and PCR instrument.The results were observed by precipitation,fluorescent reaction and electrophoresis.Results：The product of LAMP based on water-bath-heating method and PCR-instrument-heating presented more obvious precipitation,stronger fluo-rescence reaction,lighter gel electrophoresis.However,the air-bath-heating method showed much more weak results.Conclusion：The LAMP amplifications performed by water-bath-heating and PCR-instrument-heating,water bath heating were more economical choice for LAMP.Considering the cost of equipment and experimental conditions,water-bath was the preferred heating method. |
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| Keywords: | Loop-mediated isothermal amplification（LAMP） Heating method Legionella pneumophila Water bath |
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