模拟微重力诱导PC12细胞衰老的初步探讨

目的：构建模拟微重力条件下PC12细胞的培养体系,探讨模拟微重力对PC12细胞衰老的影响。方法：用Cytodex-3型微载体作为PC12细胞的贴附载体,旋转细胞培养系统所提供10-2g的微重力环境进行模拟微重力条件下的细胞培养。在倒置显微镜下观察PC12细胞的生长情况;用扫描电镜观察PC12细胞超微结构的变化;衰老相关β半乳糖苷酶（SA-β-gal）特异性染色对衰老的PC12细胞进行评估。结果：光镜下模拟微重力培养的PC12细胞表现出类衰老细胞的形态,扫描电子显微镜下观察发现其微绒毛增多。SA-β-gal染色的结果显示在模拟微重力的作用下,PC12细胞SA-β-gal的活性升高。结论：模拟微重力可以引起PC12细胞衰老样的形态变化,以及SA-β-gal的活性升高。

Influence of Simulated Microgravity on Cellular Senescence of PC12 Cells

Objective： To develop PC12 cell culture system under the condition of simulated microgravity and to investigate the influence of simulated microgravity on cellular senescence of PC12 cells.Methods： Cytodex-3 micro-carriers were used as the adherent carrier of PC12 cells.The microgravity environment was simulated by a rotary cell culture system.The growth of PC12 cells cultured on micro-carriers was detected by inverted microscope.The ultrastructure was detected by the scanning electron microscope.Senescence-as-sociated β-galactosidase（SA-β-gal） was employed to distinguish the senescent PC12 cells.Results： Simulated microgravity induced senescent-liked morphologic changes,the microvillus were abundance on simulated microgravity cultured PC12 cells.The SA-β-gal activity was upregulated by simulated microgravity culture.Conclusion： Simulated microgravity induced senescent-liked morphologic changes and upregulated SA-β-gal activity.