SD大鼠骨髓间充质干细胞体外原代培养及鉴定

目的：探讨骨髓间充质干细胞（BMSCs）体外分离培养以及扩增的方法并鉴定。方法：取100g左右雄性SD大鼠后肢股骨、胫骨骨髓,原代全骨髓培养法,多次传代纯化,体外扩增后,观察细胞形态,并免疫组化及流式细胞仪检测cd34、cd90、cd105细胞因子,鉴定是否为BMSCs。结果：所获取的细胞呈长梭形,呈现特征性的漩涡状生长,CD34阴性,CD90、CD105阳性。结论：利用全骨髓培养法成功分离骨髓间充质干细胞,10代以内的细胞纯度高,活性好。全骨髓培养较为简便、易行。

Study on Isolation,Culture and Identification of Bone Mesenchymal Stem Cells of SD Rats in Vitro

Objective：To investigate the method for isolation and amplification of the bone mesenchymal stem cells（BMSCs） in vitro and to identified it.Methods：Male SD rats around 100g,the bone marrow cells were obtained from hind femur and tibia,whole bone marrow primary culture,purified by repeated passage in vitro amplification.Cell morphology was observed,and expressions of CD34,CD90,CD105 cell factors were examined by flow cytometry,to identify whether it＇s the BMSCs.Results：Cells appeared spin-dle-shaped and showed characteristic swirling growth.The surface marker CD34 was negative,CD90,CD105 were positive.Conclusion：We successfully isolated the bone marrow mesenchymal stem cells（BMSCs） by the whole bone marrow culture method,and found that cells had high purity and good activity within 10th generation.The whole bone marrow culture was simple and easy.