哮喘患者外周血Treg和Th1/Th2的变化及其与哮喘病情的关系

目的：探讨哮喘患者外周血调节性T细胞（Treg）以及辅助性T细胞（Th1/Th2）的比例的变化,探讨其在哮喘的临床治疗中的作用。方法：80例哮喘患者（哮喘组）按临床表现分为急性发作期组（54例）和缓解期组（26例）,同时选择50例健康体检者。应用流式细胞仪检测上述各组外周血CD4＋CD25＋Foxp3＋Treg、CD4＋IFN-γ＋Th1和CD4＋IL-4＋Th2细胞水平,并进行统计学分析。结果：哮喘组CD4＋CD25＋Foxp3＋Treg水平亦明显低于正常对照组（P〈0.05。其中急性发作期组Treg水平明显低于缓解期组和正常对照组（P〈0.05）。而哮喘组Th1/Th2比值显著低于对照组（P〈0.05）,且在哮喘急性发作组中Th1/Th2比值显著低于缓解期组和正常对照组（P〈0.05）。结论：提示Treg和Th在哮喘的发生和发展中起着重要的作用。

The changes of regulatory T cells and T helper cell in peripheral blood and their significance in patients with asthma

Objective：To investigate the changes of CD4＋CD25＋Foxp3＋ regulatory T cells（Treg） and T helper cell（Th1/Th2） in peripheral blood and their significance in the severity of patients with asthma.Methods：Eighty patients with asthma（asthma group） were divided into acute attack stage group（54 cases） and remission stage group（26 cases） according to their clinical features,Meanwhile,fifty health volunteers were enrolled as control group.The levels of CD4＋CD25＋Foxp3＋ Treg,CD4＋IFN-γ＋ Th1and CD4＋IL-4＋ Th2 in periph-eral blood of all children were tested by flow cytometer（FCM）,and analyzed statistically.Results：The levels of CD4＋CD25＋Foxp3＋ Treg of patients with asthma were lower than those in the control group（P〈0.05）.The Treg levels in acute patients group were lower than those in remission group or in the control group（P 0.05）.The ratio of Th1/Th2 of patients with asthma were lower than those in the con-trol group（P〈0.05）,and The Th1/Th2 ratio in acute attack group were lower than those in remission group or in the control group（P〈0.05）.Conclusion：Tregand Th immunity plays an important role in the pathogenesis of asthma..