小鼠胰腺大部分切除后再生集中区来源的初步探索

目的：探讨胰腺在某些损伤或病理条件下,由于细胞活跃增殖产生再生集中区域的细胞来源。方法：将27只成年ICR系小鼠分为9组,每组3只,其中1组进行假手术,其余8组进行小鼠胰腺大部分切除,分别在切除后12h,24h、36h、48h、3d、5d、7d、10d取材及冰冻切片,采用H-E染色、免疫荧光染色方法检测损伤后各时间段胰腺组织的形态变化和细胞增殖率。结果：H-E染色发现,胰腺手术72h后,剩余胰腺中就出现由细胞角蛋白阳性导管样结构组成的再生集中区,此区域细胞随后分化为功能性细胞类型,10d后消失检测不到。对胰腺再生集中区的定位研究表明,它们仅出现于切除后的伤口边缘。BrdU标记表明,胰腺再生集中区为细胞快速增殖区域,其出现与总导管增殖率提高同时发生,主/大导管和小导管增殖率上升都晚于再生集中区的出现。结论：小鼠胰腺大部分切除后再生集中区可能来源于腺泡细胞的快速增殖,而不是经由总-主/大-小导管-快速增殖区这一途径引起的来源于导管上皮细胞。

Preliminary Study on the Cell Source of Focal Areas in Residual Mouse Pancreata after Partial Pancreatectomy

Objective： To investigate the cell source of focal areas in residual mouse pancreata after partial pancreatectomy.Methods： Pancreata after partial pancreatectomy was performed in ICR mouse.The rats were sacrificed at 12hours,24hours,36hours,48hours,3days,5days,7days,10days after operation respectively.H-E staining and immunofluorescence was used to detect the changes in pancre-ata cell morphology and cell proliferation rate after partial pancreatectomy.Results： The H-E staining analysis showed that there were focal areas full of cytokeratin-positive duct-like structures at 72h after surgery,then these areas differentiated rapidly and disappeared after 10 days.Localizing the focal areas in residual pancreata showed that they only situated nearby the cut edge,but not uninjured portion.BrdU labeling tests showed that focal areas were rapidly proliferating areas,and they appeared simultaneously with the increase of common ducts cell proliferation.The focal areas was earlier than the increase of main/large and small ducts cell proliferation.Conclusion： Focal areas in residual mouse pancreata maybe come from the rapid proliferation of acinar cells,but not from the common ducts-main/large ducts-small ducts-focal areas pathway in partial pancreatectomized mice.