氯化钴对H9c2心肌细胞新基因Mipu1表达的影响

目的：研究氯化钴（CoCl2）对大鼠胚胎心脏来源的H9c2心肌细胞中新基因Mipu1表达的影响。方法：利用不同浓度的CoCl2（0、100、200、300、400、500μmol/L）处理H9c2细胞9h,及200μmol/L CoCl2处理H9c2细胞不同的时间（0、6、9、12、24h）后,用RT-PCR和Western Blot分别观察H9c2细胞Mipu1 mRNA和蛋白的表达情况。结果：CoCl2可以诱导H9c2细胞中Mipu1 mRNA和蛋白表达升高,200μM CoCl2处理组的Mipu1的表达水平高于100μM CoCl2处理组,但是更高浓度的CoCl2（〉200μM）不能使Mipu1的表达进一步升高。随着CoCl2作用时间的延长,Mipu1的表达逐步升高,在12h达到高峰,但是在24h后下降。结论：CoCl2能够促进H9c2细胞新基因Mipu1的表达,并且具有一定的剂量和时间依赖性。

Effects of Cobalt Chloride on the Expression of a Novel Gene Mipu1 in H9c2 Cardiomyoblast

Objective： To investigate the effect of Cobalt chloride（CoCl2） on the expression of a novel gene Mipu1 in H9c2 rat cardiomyocytes.Methods： Various concentration of CoCl2（0,100,200,300,400,500μmol/L）was used to treat H9c2 cells for 9h,and 200μmol/L CoCl2 was used to treat H9c2 cells for various periods of time（0,6,9,12,24h）,then RT-PCR and Western Blot were used to observe the expression of Mipu1 in H9c2 cells.Results： The expression of Mipu1 mRNA and protein was enhanced by CoCl2 in H9c2 cells.Compared with the group of 100μmol/L CoCl2,the expression of Mipu1 was higher in the group of 200μmol/L CoCl2.However,the expression of Mipu1 did not improve treated by more higher concentration of CoCl2（200μM）.With the time of CoCl2 treatment ex-tending,the expression of Mipu1 gradually increased,and reached peak at12h,but decreased at 24h.Conclusion： Treatment of H9c2 rat cardiomyocytes with CoCl2 resulted in a limitedly dose-and time-dependent increase in a novel gene Mipu1 expression.