| 不同染色技术和双色荧光原位杂交技术对宽翅菘蓝（Isatis violascens Bunge）的细胞遗传学分析 |
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| 引用本文： | 戚大石,汤佳立.不同染色技术和双色荧光原位杂交技术对宽翅菘蓝（Isatis violascens Bunge）的细胞遗传学分析[J].生物磁学,2011(3):485-488. |
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| 作者姓名： | 戚大石 汤佳立 |
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| 作者单位： | [1]徐州医学院,江苏徐州221121 [2]中国药科大学生命科学与技术学院,江苏南京210009 |
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| 摘 要： | 为了解十字花科菘蓝属植物宽翅菘蓝（宽翅菘蓝,Isatis violascens Bunge）的染色体结构,本文利用45SrDNA和5SrDNA双色荧光原位杂交、银染技术和CMA3对宽翅菘蓝进行分子细胞遗传学研究。45SrDNA和5SrDNA荧光原位杂交结果显示宽翅菘蓝染色体上有1对45SrDNA信号和1对5SrDNA信号;银染结果显示其中期染色体有1对银染点;CMA3染色结果发现宽翅菘蓝中期染色体存在1对CMA3信号。
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| 关 键 词： | 宽翅菘蓝 银染 45SrDNA 双色荧光原位杂交 CMA3染色 |
Cytogenetic Analysis of Isatis Violascens Bunge by Different Staining Techniques and D-Fluorescent in Situ Hybridization |
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| QI Da-Shi,TANG Jia-Li.Cytogenetic Analysis of Isatis Violascens Bunge by Different Staining Techniques and D-Fluorescent in Situ Hybridization[J].Biomagnetism,2011(3):485-488. |
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| Authors: | QI Da-Shi TANG Jia-Li |
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| Institution: | 1 Xuzhou Medical college,Xuzhou,221116,China; 2 School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China) |
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| Abstract: | Objective In order to understand the chromosome structure of I.violascens Bunge,45S rDNA and 5S rDNA dual-flu-orescence in situ hybridization（45S rDNA and 5S rDNA D-FISH）,silver staining and CAM3 staining were used to study the chromo-somes of I.violascens Bunge.In the silver stained metaphase chromosomes of I.violascens Bunge,there were two positive regions;fluo-rochrome Chromomycin A3（CMA） staining showed two positive regions;D-FISH with 45S rDNA and 5S rDNA probes revealed one 18S-5.8S-26S rDNA loci and one 5S rDNA loci with different intensity. |
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| Keywords: | Isatis violascens Bunge Silver staining 45S rDNA D-fluorescence in situ hybridization CMA3 staining |
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