Strong suppression of chloramphenicol acetyltransferase expression by inducing T7 RNA polymerase |
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Authors: | Han Bok Kim Changwon Kang |
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Affiliation: | (1) Department of Life Science, Korea Advanced Institute of Science and Technology, 373-1 Kusong-Dong, Yusung-Gu, 305-701 Taejon, Korea |
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Abstract: | Summary Suppression of gene expression can be used as a way to study gene regulation. Expression of chloramphenicol acetyltransferase (CAT) underE. coli promoter was inhibited by 92 % by induction of T7 RNA polymerase which transcribes from a T7 promoter opposingE. coli promoter. |
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